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KMID : 0378019690120080073
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New Medical Journal
1969 Volume.12 No. 8 p.73 ~ p.80
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Effect of fracture and Sex Hormones on oxidative metabolism and turn-over rate of citrate in bone
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Abstract
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Forty male rats, weighing approximately 200 gm were divided into 4 groups, the first group for control, the second group for estrogen injected group, the third for androgen injected group and the fourth for progesterone injected group.
The left femur of every rat of each group was fractured by manual clasis before administration of sex hormones and in sex hormone administered groups, 25 mg of respective sex hormone was injected subcutaneously into the rat for 3 days.
The rat of each group were slaughtered next day after final injection of sex hormone and both femora of rats were freed of soft tissue and homogenized in the incubation media containing C´° citrate. Bone honogenates were incubated in the Dubnuff shaking metabolator for 3 hours.
After final period of incubation, CO2 samples, which were trapped as Nat CO3 in the incubation flask, were analyzed for their radioactivity and concentration and radioactivities of bones were measured for the calculation of turn-over rates of bone citrate.
Results were as follow.
1) Total CO2 production rates and RSA were increased by fracture in each group and accelerated by sex hormones.
2) Blood levels of citrate were mean of 13.4±4.12/ml in the control group, 13.1±2.4 in the estrogen administered group, 21.0±5.2 in the androgen administered group and 15.0±2.52/ml in the progesterone administered group. Blood level of citrate was generally increased by sex hormones.
3) The concentration of bone citrate was mean of 3.67±1.10mg/gm which showed more than 200 times of blood level bone contents of citrate were increased by fracture and accelerated by sex hormone administration.
4) The turn-over rate of bone citrate averaged 0. 75% /hr in the non fractured control bone. These values in the fractured bones in each group showed always higher than that of non fractured bone and accelerated by sex hormone administration.
The half time(T 1/2) of turn-over of citrate accordingly decreased by frcature and sex hormone. In summary: Fracture and sex hormone stimulated the addidative metabolism of bone, especially that of citrate. The fact that turn-over rates of citrate increased by fracture and sex hormone suggested increased mobilization of Ca.
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KEYWORD
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